7/31/2023 0 Comments Klenow fragment ligation![]() Intriguingly, XRCC1 has been shown to associate and co-localize with PCNA during the S phase ( 23, 24), but other studies have concluded that poly (ADP-ribose) (PAR) synthesis is important for LIG3-XRCC1 recruitment at chromosomal DNA break sites ( 25–27). The question arises as to how LIG3 is specifically localized to DNA replication sites in the absence of LIG1 during S phase progression because the domain structures of LIG1 and LIG3 are very different, especially given that LIG3 lacks a PIP-box. Systematic studies using a chicken B cell line demonstrated that even LIG1/LIG4 double knockout cells are viable ( 16), suggesting that LIG3 may function in DNA replication in the absence of LIG1. It has also been suggested that LIG3 might be responsible for alternative Okazaki fragment ligation in the absence of LIG1. Nuclear LIG3 forms a tight complex with its partner protein X-ray repair cross-complementing protein 1 (XRCC1) and is involved in nucleotide excision repair (NER), single-strand break repair (SSBR), and base excision repair (BER) ( 7, 20–22). LIG3 is conserved in vertebrates and also in some lower eukaryotes ( 19). Unlike yeast, however, LIG1 is not essential for cell survival ( 15–18), implying the existence of a compensatory pathway other than that of LIG1. In vertebrates, LIG1 also localizes at DNA replication foci and functions as the main DNA ligase responsible for Okazaki fragment joining ( 11, 14). Cdc9 physically interacts with PCNA via its conserved PCNA interacting peptide (PIP) box motif at the N-terminus and localizes at sites of DNA replication, catalyzing the ligation of Okazaki fragments ( 11–13). In budding yeast, the CDC9 gene, a homolog of human LIG1, is essential for viability ( 9, 10). LIG4 plays an essential role in non-homologous end-joining (NHEJ), the pathway for DNA double-strand break repair (DSBR). LIG1 is the major ligase functioning in DNA replication, and also acts in a fusion of sister chromatids by targeting double-stranded DNA breaks ( 8). Vertebrates have three different types of DNA ligases, LIG1, LIG3 and LIG4 ( 7). Therefore, ligation of Okazaki fragments by DNA ligase must be strictly controlled to reliably maintain genome stability. Lagging strand synthesis is completed by joining millions of Okazaki fragments through the action of DNA ligases, and a failure of Okazaki fragment joining results in an enormous amount of gap/nick formation on genomic DNA. The displaced 5′ flap is cleaved by nucleases, such as FEN1 or DNA2, and generates a ligatable nick ( 4–6). When DNA polymerase delta (POL δ) reaches the end of a nascent Okazaki fragment, it generates a 5′ flap structure by displacing the 5′ end of the preceding fragment. Lagging strand synthesis is a tightly coordinated stepwise reaction involving multiple proteins, including PCNA, which is an essential component of DNA replication ( 1–3). Our results suggest that a unique form of ADP-ribosylation signaling promotes the recruitment of LIG3 on chromatin and its mediation of Okazaki fragment joining as a backup system for LIG1 perturbation. Importantly, Okazaki fragments were not ligated at all when LIG1 and XRCC1 were co-depleted. The depletion of PARP1 or HPF1 resulted in a failure to recruit LIG3 onto chromatin and a subsequent failure in Okazaki fragment joining in LIG1-depleted extracts. Concomitantly, LIG1 deficiency induces ADP-ribosylation of histone H3 in a PARP1-HPF1-dependent manner. ![]() We found that Okazaki fragments were eventually ligated even in the absence of LIG1, employing in its place LIG3-XRCC1, which was recruited onto chromatin. Here, using a cell-free system derived from Xenopus egg extracts, we demonstrated the essential role of PARP1-HPF1 in LIG3-dependent Okazaki fragment joining. Recent studies have implicated LIG3 complexed with XRCC1 as an alternative player in Okazaki fragment joining in cases where LIG1 is not functional, although the underlying mechanisms are largely unknown. DNA ligase 1 (LIG1) is known as the major DNA ligase responsible for Okazaki fragment joining.
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7/31/2023 0 Comments Photo capturing a web pageThe tool allows you to select any area on your desktop and capture it as a screenshot with the click of a button. The easy to understand interface lets you work with the application easier and faster. Lightshot is an easy to use application with a simple and intuitive user interface. In case you want to capture only the visible part of the page, you may do that too. You can upload these captured screens to OneNote, clipboard or email and print them for future reference. You may even edit and annotate captures and save them as PDF/JPEG/GIF/PNG files. FireshotįireShot is an extension available on the Chrome Webstore that lets you capture full web page screenshots. You may capture your entire desktop, region, window, or even a scrolling screen without any hassle. ![]() ![]() Apart from that, the tool makes it incredibly easy for you to edit your screenshot or record video. It lets you quickly create high-quality images and videos and takes your screen capturing experience to the next level. The software supports built-in advanced image editing and screen recording. TechSmith is a simple and easy-to-use tool for capturing screenshots. Rather than using conventional tools that let you screenshot a page across one browser at a given time, consider switching to modern tools like LambdaTest that enable you to capture instant screenshots across multiple browsers in a single click. Although it is a cross-browser testing tool that allows you to perform cross-browser testing on more than 2000 real browsers and operating systems online, it also lets you capture auto-generated full-page screenshots of your web pages across multiple devices, operating systems, browsers, and resolutions in a single click to perform visual cross-browser testing. LambdaTestĪ lot of budding platforms like LambdaTest have inbuilt screenshot capturing support. Apart from that, use the tool to record all the ongoing screen activities and much more. Whether it is a scrolling windows/web page or a fixed length area, you can capture it with ease using FastStone Screen Capture. The platform also supports screen video recording and lets you quickly capture and elucidate anything on the screen. FastStone Screen CaptureįastStone Capture is a popular tool used for capturing website screenshots. Keeping that in mind, here we have brought you the top 10 tools to take full page website screenshots for various purposes. From taking a screenshot of a funny meme for sharing with your friends to capturing screenshots of web pages for analyzing them during visual cross-browser testing, screenshots are used almost everywhere.Īlthough we all are familiar with conventional methods of capturing screenshots like Snipping Tool, not many of us are well-versed with the latest tools available in the industry to get the job done. ![]() However, the situation has changed a lot today with screenshots becoming a routine affair. They were not that much into popular culture and had a limited application. Until a few years ago, screenshots used to be rarely used. ![]() ![]() The diagram depicts an object launched upward with a velocity of 75.7 m/s at an angle of 15 degrees above the horizontal. A vertical force causes a vertical acceleration - in this case, an acceleration of 9.8 m/s/s.īut what if the projectile is launched upward at an angle to the horizontal? How would the horizontal and vertical velocity values change with time? How would the numerical values differ from the previously shown diagram for a horizontally launched projectile? The diagram below reveals the answers to these questions. This is indeed consistent with the fact that there is a vertical force acting upon a projectile but no horizontal force. This is to say that the vertical velocity changes by 9.8 m/s each second and the horizontal velocity never changes. The numerical information in both the diagram and the table above illustrate identical points - a projectile has a vertical acceleration of 9.8 m/s/s, downward and no horizontal acceleration. These same two concepts could be depicted by a table illustrating how the x- and y-component of the velocity vary with time. The important concept depicted in the above vector diagram is that the horizontal velocity remains constant during the course of the trajectory and the vertical velocity changes by 9.8 m/s every second. The lengths of the vector arrows are representative of the magnitudes of that quantity. If a vector diagram (showing the velocity of the cannonball at 1-second intervals of time) is used to represent how the x- and y-components of the velocity of the cannonball is changing with time, then x- and y- velocity vectors could be drawn and their magnitudes labeled. This means that the vertical velocity is changing by 9.8 m/s every second. Yet in actuality, gravity causes the cannonball to accelerate downwards at a rate of 9.8 m/s/s. If there were no gravity, the cannonball would continue in motion at 20 m/s in the horizontal direction. Suppose that the cannonball is launched horizontally with no upward angle whatsoever and with an initial speed of 20 m/s. As you proceed through this part of Lesson 2, pay careful attention to how a conceptual understanding of projectiles translates into a numerical understanding.Ĭonsider again the cannonball launched by a cannon from the top of a very high cliff. You will learn how the numerical values of the x- and y-components of the velocity and displacement change with time (or remain constant). In this portion of Lesson 2 you will learn how to describe the motion of projectiles numerically. The horizontal motion of a projectile is independent of its vertical motion.The vertical velocity of a projectile changes by 9.8 m/s each second,.There is a vertical acceleration caused by gravity its value is 9.8 m/s/s, down,. ![]() The horizontal velocity of a projectile is constant (a never changing in value),.There are no horizontal forces acting upon projectiles and thus no horizontal acceleration,.Projectiles travel with a parabolic trajectory due to the influence of gravity,.A projectile is any object upon which the only force is gravity,.So far in Lesson 2 you have learned the following conceptual notions about projectiles. 7/31/2023 0 Comments Imac big screen![]() Next, you’ll see a list of AirPlay-compatible devices that your Mac has discovered on your network. When Control Center appears, click “Screen Mirroring.” To share your screen using AirPlay on a Mac running macOS 11 Big Sur or later, first, click the Control Center icon located in the upper-right corner of your screen (in the menu bar). RELATED: How to Screen Mirror on Roku Share Your Screen on macOS 11 Big Sur and Later Compatible devices include Apple TV, certain brands of smart TV sets, and other devices running AirPlay receiver software. Michael Potuck 2:01 pm PT One of the new health features for iPhone and iPad with iOS 17 can help prevent eye strain for everyone, as well as lower the risk of myopia. Chromecast: What's the Difference? You’ll Need an AirPlay-Compatible Receiving Deviceīefore we begin, we’re going to assume that you already have at least one AirPlay-compatible receiver device connected to the same local network as your Mac. That’s what we’re going to explore in this article. nanoleaf Matter Nanoleaf launches immersive 4D TV Screen Mirror and Lightstrip Kit with Matter Michael Potuck 9:50 am PT 0 Comments Back at CES in January, Nanoleaf announced a. One of the most useful features of AirPlay is that you can use it to share (project) your Mac’s screen onto another display device. with minor alterations to screen size or aspect ratio being the most noticeable updates. Apple currently offers the 24-inch iMac featuring the. The 24-inch iMac has a colorful and thin design. 5) Double click on the renamed file to start its installation. 4) Rename the copied file to something such as Hey, while keeping the. 3) Find the Hello.saver file and copy it to your Desktop. That’s estimated to leave room for a 6.4-inch display, which would sit right in between the S23 and S23+. 1) In Finder, hold down the Option key and click on Go. ![]() A bigger iMac would be the perfect desktop computer for those dreaming about the Mac Studio + Studio Display combo but can’t afford it. As far as the size goes, the phone measures 158 x 76.3 x 8.2mm. It’s similar to how Miracast and Chromecast work on Windows and Android devices. Apple has reportedly kicked off early development work on a new desktop computera bigger iMac with a screen measuring over 30 inches diagonally. Apple iMac 21.5 Desktop 1.4GHz Core i5 8GB macOS 11 Big Sur Tested/Works Reset. AirPlay lets Apple devices such as Macs, iPhones, and iPads stream audio and video over a local network to an AirPlay-compatible receiver device without the need for audio or video cables. Apple 24 iMac w/ Retina 4.5K display M1 8GB Memory 256GB SSD Green. Will its bigger sibling also do away with the convex back Sarah Tew/CNET. ![]() AirPlay is Apple’s name for the company’s proprietary wireless media streaming protocol. PT 4 min read The 24-inch M1 iMac redesign brought a slim, flat, iPad Pro-like silhouette. 7/31/2023 0 Comments Steam id finder lionIf you are “game-banned” - you just can’t join multiplayer in this game. You may get this ban in many different ways, among them cheating, offensive language, and other game-specific violations. Game ban in Steam defines a ban from multiplayer games that don't use VAC to detect player violations. SteamID checker allows to find bans and restrictions Game bans Let’s break down “bans and restrictions” into details.
7/31/2023 0 Comments Best way to kill reaper leviathan![]() Finally, I pressed the only button that would work and left the Seamoth, making my worst fears come true as the Reaper targeted me instead. I was too busy crying (and also didn’t know I was supposed to do anything) to get out of my Seamoth, so the Reaper inflicted a massive amount of damage to it. I hadn’t even finished that thought when the hulking behemoth of a leviathan snuck up behind me, grabbed my minuscule Seamoth, and throttled it in between its massive jaws, scaring me so badly that I literally jumped in my seating and began dry-sobbing. Instead, I was faced with a sizable bit of machinery. Deciding that I no longer wanted to partake in this thing’s game of deadly marco-polo, I turned to leave, expecting to face the biome’s edge. To put that into perspective, that’s half the length of a football field (which is 360 feet), around ten times the size of the average car, and definitely bigger than your tiny human body.Īlthough curiosity was getting the best of me, I managed to fight off the urge to interact with such a creature–especially when I went closer to the creature’s last known location (literally right in front of me, barely outside of my murky vision), and the beast was gone. Reaper Leviathans are 55 meters long, which translates into a length of 180 feet. That’s when I saw it: the mighty Reaper Leviathan. Unfortunately for me, I never looked to my left, so instead of being on the edge of the Crash zone, I was directly in the middle of it. I didn’t even know I was in the Crash zone, either! I thought I was in the Dunes! Granted, the Dunes are dangerous as well, but I was keen on staying on the edge of said biome. If you’ve seen any content about the Crash zone, you’ll probably immediately cringe at the fact that I went in there unprepared. The Aurora Crash zone is a minefield (metaphorically). Since I somehow thought I was in the Dunes, I continued through the Crag field and went into the Crash Zone. I made a left, taking me into the Crag Field. I wanted to go to the Dunes, which would be to the right of the floating islands. After going to a landmark (the floating islands), I made a disastrous mistake, though. I did a bit of an internet search and found a map of the game’s land. However, on this occasion, I decided that I would go adventuring. I am usually cautious in Subnautica: I don’t want to die (even though, from a game play standpoint, dying has one of the nicest setbacks I’ve seen in a survival series), so I play very safely. Having said that, exploration is very dangerous, and the further you get out from your starting point, the more danger you’ll encounter. As a survival exploration game, the game is all about surveying your surroundings to find ways to stay alive and improving your quality of life. ![]() Some are safe to be around, most want to kill you, and they all impact your gameplay in some way. Subnautica takes place on an alien planet named 4546B, a “land” (or, well, gigantic body of water) full of unknown creatures. ![]() The game is good for giving its fans newfound fears of the ocean that much I can say for sure. ![]() It’s a stunning game, which can switch from “beautiful surroundings I’m glad to be a part of” to “I need an extra change of pants and a hug” on a coin flip. If you’ve never heard of Unknown Worlds Entertainment (a fitting name for the company when you look at its recent game series), I would recommend checking out a lovely horror game: Subnautica. ![]() Once you've installed Discord on your computer, click the giant + symbol on the left menu, then click "Join a Server"and paste the invite link.A full-on sequel to Horizon Zero Dawn, Forbidden. Join Aloy as she braves the Forbidden West a majestic but dangerous frontier that. Its nearly here: Horizon Forbidden West is set to release on PS5 and PS4 on Febru( see it on Amazon / get it in the UK ). If you're new to Discord, you can get started by downloading the official Discord client from here. Horizon Forbidden West - Preorder Bonus (PS4, PS5) - PSN Key - EUROPE.This edition comes with a physical copy of the game, steelbook case, mini art book, and digital. Horizon Forbidden West PS4 Buy and Get FREE DLCs 6294016542096 is Action, Adventure Game published by Sony Interactive Entertainment, Buy Online Fast. ![]() We want a healthy balance of images and discussion, so we will start removing posts if the front page is flooded with images.ĬHECK OUT THE FULL RULES HERE Discord Server Horizon Forbidden West's Special edition is available to preorder for 70 on PS4 or 80 on PS5. 5) Do not go overboard with image posts.After pre-ordering Horizon Forbidden West: Burning Shores, you will get access to both the exclusive Blacktide Dye Outfit and the Blacktide Sharpshot Bow. Criticism is okay but remember, everyone will have their own opinion on a certain topic. Horizon Forbidden West PS4 Confront new dangers, Engage in strategic battles against enormous machines and mounted human Unravel startling mysteries. You can currently pre-order Horizon Forbidden West’s Burning Shores post-game expansion by heading to its page on the PlayStation Store and then purchasing it for 19.99. Physical preorders are live now at major. ![]()
7/31/2023 0 Comments National guard bus driversThis nursing shortage mirrors others nationwide where nurses and other medical staff have quit or been fired over vaccine mandates.Īs President Biden attempts to interfere with the private sector by ordering all companies with 100 or more employees to require vaccination or frequent testing, the labor shortage in many sectors is likely to get even worse.Īs for parents, the school bus driver shortage is just the latest example of how government schooling is failing many students and families. My colleague Jon Miltimore wrote this week about one hospital in upstate New York that had so many nurses quit over the vaccine mandate, it announced it will have to stop delivering babies this month. Indeed, new COVID-19 vaccine mandates are causing workers in many fields to quit their jobs. In Chicago, more than 70 school bus drivers quit just before the school year began as a result of the district’s new COVID-19 vaccine mandate for all employees. Vaccine mandates have been a key factor in the school bus driver shortage. Some school districts are actually paying parents to drive their own children to school. Pittsburgh Public Schools had to push back their first day of school by two weeks due to the driver shortage, while high school students in Rochester, New York had to begin the year remotely due to the lack of bus drivers. The school bus driver shortage has caused major disruptions to the start of the school year. Currently, 90 National Guard troops are being trained as school bus drivers in the state, with up to 250 troops involved in the overall mission. Here in Massachusetts, the school bus driver situation is so bad that this week the governor called up the National Guard to drive school buses in some of the state’s urban areas that are experiencing the worst driver shortages. ![]() ![]() ![]() Districts have been scrambling to hire new bus drivers, including offering generous perks and bonuses, but these efforts aren’t making much of a dent in attracting new employees. According to The Wall Street Journal, many school bus drivers quit over virus concerns or new COVID-19 vaccine mandates. Click here to sign up.Īs the new academic year begins, a school bus driver shortage has impacted students and families all across the country. This article is excerpted from LiberatED, a weekly email newsletter where FEE Senior Education Fellow Kerry McDonald brings you news and analysis on current education and parenting topics. 7/31/2023 0 Comments National unity synonymIn contrast to many other languages with grammatical aspect, the distinctions borne by pfv vs ipfv aspect are based on patterns of stem derivation marked by prefixes and suffixes. This does not imply that the system across Slavic languages is identical in all of its parts, but we can say that its fundamental architecture is the same for all Slavic languages. The opposition of perfective (pfv): imperfective (ipfv) aspect can be considered a ‘proprietary label’ of Slavic languages as a whole, inasmuch as in all contemporary and historically attested Slavic languages the core of their aspect system is organized in an identical manner, both concerning basic morphological patterns and basic functional distinctions. Our findings, as well as a revision of theoretical positions, support a notion of paradigm in which typical inflectional distinctions are brought into an equilibrium with functional inventories and collocational constraints. These preferences prove stable over time, distinctions in form typically associated with inflection turn out to be altogether irrelevant. The two ipfv stems show preferences for different basic functions associated with the ipfv aspect, the coding of marginal arguments and adjuncts also yields clear biases of choice. We provide their token-based analysis for the period 1750–2017. On this background, we test some core assumptions of the morphology-lexicon interface on one typical aspect triplet from Polish and Czech, the cognate ipfv Pol. However, can derivationally related stems claimed to represent identical lexical concepts be treated as representatives of one or of two paradigms? The issue becomes especially intricate with aspect triplets in which two ipfv stems correspond to one pfv stem, as though combining two productive patterns of aspect derivation. It creates a complex network of functions for finite and non-finite forms, which largely applies regardless of aspectual pairedness (and actionality groups), since this opposition has classificatory properties. ![]() The Slavic perfective (pfv): imperfective (ipfv) opposition is based on stem derivation. 7/31/2023 0 Comments Lynda carter invisible jet![]() Q: Okay, so it sounds like you've got wide-ranging musical taste - is there anything in your music collection that's embarrassing?Ī: If you looked at my vinyl collection? Maybe - but even something cheesy like (singing) "It's My Party and I'll Cry if I Want To" is still good. If he wants to go on a ski vacation with guy friends, I say, "Go, have fun!" And if I want to visit family or vacation with girlfriends, he says, "Go, have fun!" And we're totally supportive of each other. (Washington attorney Robert Altman, who is also the chief executive of a Rockland, Maryland-based company that makes video games.) I also have eight original songs on the album, including a country song called "After All These Years" about my husband of 34 years. It's in keeping with my very eclectic taste in music - I do everything from Billie Holiday to the Black Keys to James Taylor. You wouldn't think of me singing this song, but people like it, and we love doing it live. So we added this song - it's called "Put the Gun Down" by ZZ Ward, and it's this very bluesy hard rock. And I did a few new songs, and he turns to his friend and goes, "wait, why aren't we doing that one on the album?" Q: So tell me about the album you're working on.Ī: We had finished it, and we were doing final touches, and then I did a (live performance) in Nashville, and my producer was there. ![]() We have to start spreading that news - that real men change poopy diapers and coach the soccer team and let their daughters paint toenails all kinds of colors. ![]() ![]() It's a statement, but I think we need to start spreading the news about a feminism that doesn't marginalize the men in our lives. Q: What do you make of these women-only screenings of the movie?Ī: I think they're fabulous! That anyone would whine about it - they are extra screenings they are not excluding anyone. You know she had to fight for every little thing to keep her vision honest. Who's going to watch that?'Īnd in some ways, we've taken a step back - Patty didn't tell me this, but I just know that it wasn't easy to make this movie. I was just writing an email to Patty about this - more than 40 years ago they told me, 'we're gonna do a pilot, but honestly, a woman won't be able to sustain an hour of TV. Q: Do we need Wonder Woman again at this moment?Ī: Timing-wise, it's perfect. But there's a perspective that comes from being famous for it. I mean, so does anyone who ever believed in herself as Wonder Woman, whether it was a little girl or a gay boy - they know, too. The three of us really understand what being on the inside of Wonder Woman's skin feels like. And then when I met Gal, I linked the other arm with her. Q: You played that character for a long time, and you're so closely identified with her - what was it like seeing someone else play her?Ī: When it was first given a green light, it would be dishonest of me to say I didn't have a little pang of "well, here it goes." But after my first conversation with Patty, I felt I had linked arms with a woman who I would go though the rest of my life with. You and I are her, and that's why you see those little signs and T-shirts that say things like "Wonder Woman works here" or "My mom is Wonder Woman." Patty was able to bring that all to the screen. There's beauty and strength that transcend time and events. It isn't about a caper - no pun intended - but it's more about who Wonder Woman is inside, and the people who understand how I played her understand that.Ī: She is strong, and she can do anything, and there's this goodness to her. Patty Jenkins was able to convince people that her vision was the right vision. Q: There's been a big gap between when you played Wonder Woman and now - why did it take so long to bring this iconic character to the big screen?Ī: They needed the voice of a strong female director who got it. Since Carter retired her invisible plane, she's gone on to voice work for video games, produced three albums (she was a singer/songwriter before she started acting), and played plenty of other roles, including the president of the United States on the TV show "Supergirl." We caught up with the Potomac, Maryland, resident while she wrapped up recording sessions in Nashville for her latest album and prepared to head back to Washington. The actress played the superhero and her alter ego, Diana Prince, on the TV version that aired from 1975 to 1979. Though it looks to be a hit and a potential franchise-in-the-making, for some people, Wonder Woman will always be Lynda Carter. The big-budget "Wonder Woman" starring Gal Gadot as the lasso-twirling Amazon princess, hits theaters Thursday. |
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